Stimulation of Wound Revascularization by Adenosine Receptor Activation - Extracellular ATP and Adenosine as Regulators of Endothelial Cell Function - page 96

Fig. 6.2 Effect of inflammatory cytokines on the expression of G β 1 and G β 4 proteins in HMVEC.
Cells were treated overnight with IL-1, TNF- , followed by mRNA and protein analyses.

Quantitative real-time PCR was performed to estimate the copy number of cDNA for G β 1 and G β 4
proteins, and data are expressed as the percentage of GAPDH cDNA. Data shown are the mean
(

α ,orIFN- γ

SEM) of four independent experiments. ∗ p < 0.05; ∗∗ p < 0.01. Reproduced from [42] with
permission.

±

Fig. 6.3 TNF- differential modulation of the stimulatory effect of the adenosine

receptor agonist CGS-2168 on VEGF mRNA expression. HMVECs were pretreated overnight
with TNF-

α and IFN- γ

α or IFN- γ , then stimulated with CGS-21680 (1 μ M) for 4 h in the absence (CGS)

or the presence of 1 M ZM-241385 (CGS + ZM). The expression of VEGF message was quan-

titated by real-time PCR and is presented as a percentage of the control (none). The basal levels
of VEGF in unstimulated cells (without agonist or antagonist) were 1.55

μ

± 0.30, 2.02 ± 0.59, and

1.62 ± 0.12% of GAPDH mRNA for control, TNF- α -treated, and IFN- γ -treated cells, respectively

( n = 3). Reproduced from [42] with permission