Biology Reference
In-Depth Information
Fig. 6.1
cAMP accumulation in cytokine-treated dermal human microvascular endothelial cells
(HMVEC). HMVECs were treated overnight with TNF-
and thereafter stimulated with
the A
2A
adenosine receptor agonist MRE0094. The cellular cAMP content of treated HMVEC was
measured by ELISA. (
a
) Basal cAMP levels in cytokine-treated HMVEC (without agonist stim-
ulation). (
b
) Cytokine-pretreated HMVECs were stimulated with 1
α
or IFN-
γ
M MRE0094 for various
times as indicated. (
c
) Cytokine-treated HMVECs were stimulated for 10 min with the indi-
cated concentrations of MRE0094. (
d
) Enhanced cAMP accumulation in TNF-
μ
-treated HMVEC
by the agonist MRE0094 was attenuated in the presence of antagonist ZM-241385 (10
α
μ
M).
Search WWH ::
Custom Search