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Fig. 6.1 cAMP accumulation in cytokine-treated dermal human microvascular endothelial cells
(HMVEC). HMVECs were treated overnight with TNF-
and thereafter stimulated with
the A 2A adenosine receptor agonist MRE0094. The cellular cAMP content of treated HMVEC was
measured by ELISA. ( a ) Basal cAMP levels in cytokine-treated HMVEC (without agonist stim-
ulation). ( b ) Cytokine-pretreated HMVECs were stimulated with 1
α
or IFN-
γ
M MRE0094 for various
times as indicated. ( c ) Cytokine-treated HMVECs were stimulated for 10 min with the indi-
cated concentrations of MRE0094. ( d ) Enhanced cAMP accumulation in TNF-
μ
-treated HMVEC
by the agonist MRE0094 was attenuated in the presence of antagonist ZM-241385 (10
α
μ
M).
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