Biomedical Engineering Reference
In-Depth Information
basement membrane zone of skin.
113
Correction of the JEB phenotype can be
achieved by gene transfer of LAMB3, which encodes the beta 3 subunit of laminin-
5.
114
Organotypic cultures prepared with LAMB3-transduced cells show normal
assembly of dermal-epidermal attachment structures indicating a correction of the
mutant phenotype.
115
These studies show the feasibility of combining tissue
engineering with gene therapy to treat cutaneous diseases.
12.8
Conclusions
Technological advances in the culture of skin cells have permitted the fabrication
and testing of engineered skin substitutes. Continued research will be needed to
identify more efficient methods of utilizing precious autologous tissue, provide
greater amounts of skin substitutes for grafting, and shorten the time required for
their preparation. Additional research is aimed at improving the anatomy and
physiology of skin substitutes, working toward better homology with native skin
autograft. These efforts will lead to enhanced performance of engineered skin
graft, greater clinical efficacy and a reduction of morbidity and mortality for
patients with burn injuries and other skin wounds.
12.9
References
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