Chemistry Reference
In-Depth Information
Virus-free
Solanum tuberosum
L. microplants
positive (+) and positive (-) phytoplasma 28-32
days old
Long term effect of SA
Short term effect of SA
Single node cuttings
in vitro
(+ and -)
phytoplasma infected plants were
cultivated in medium added with SA (0,
0.1, 0.01 and 0.001 mM)
Twenty to twenty five
in vitro
(+ and -)
phytoplasma plants 28-32 days old
were transplanted to greenhouse in
pots containing peat-moss
Twenty to twenty five
in vitro
(+ and -)
phytoplasma plants SA-preincubated
28-32 days old were transplanted to
greenhouse in pots containing peat-moss
(+ and -) phytoplasma plants were sprayed
with SA (0, 0.1, 0.01 and 0.001 mM) from
15 days after transplant (DAT) up to 90
days.
Evaluated symptoms were: plant height, stem thickness and length of the 6
th
internode from the base, percentage of minituber browned flesh, and the tuber
number and weight per plant
Hydrogen peroxide and ascorbate (AA) contents, and peroxidase
activity were quantified in treated and control plants at 30, 60 and 90
DAT.
Fig. 1 Schematization of methodology for short and long term effects of SA, in phytoplasma-
infected potato plants
and 0.001 mM pH 5.6-5.7, from 15 days after transplant (DAT) up to 90 days.
Positive and negative controls were sprayed with distilled water (pH 5.6-5.7;
Fig.
2
).
Plants were maintained under greenhouse conditions for 95 days and each pot
corresponded to an experimental unit. All plants were fertilized each 15 days and
watered twice a week.
2.3 Symptom Analysis
Ninety DAT, stem and tuber symptoms were analyzed for 10 plants of each
experimental unit. The evaluated symptoms were: plant height, stem thickness and
length by the 6th internode from the base, percentage of browned mintuber flesh,
and the tuber number and weight per plant.
Search WWH ::
Custom Search