Biology Reference
In-Depth Information
Table 4.1.
Conditions of
in vitro
digestion.
Digestive process
Reagent
pH
Time
References
Stomach
Small intestine
Porcine pepsin
Glicodeoxicolate
Taurodeoxicolate
Taurocolate
Porcine pancreatin
Cholesterol esterase
2.0
5.3-7.4
1 h
2.5 h
(Garrett,
et al
., 1999;
Aherne
et al.
, 2009, 2010)
Stomach
Small intestine
Pepsina
Pancreatin
Bile salts
Dialysis membrane
2.8
6.5
2 h
2 h
(Argyri
et al
., 2009, 2011)
Stomach
Small intestine
Pepsin
Pancreatin
Bile
Sodium cholate
Sodium deoxicholate
Dialysis membrane
2.0
7.5
2 h
2 h
(Gil-Izquierdo
et al.
, 2001;
Bermudez-Soto
et al.
,
2007)
Stomach
Small intestine
Porcine pepsin
Porcine pancreatin
Lipase
Bile
3.0
6.0-6.9
1 h
2 h
(Bhagavan & Chopra,
2007; Ercan & El, 2011)
Stomach
Small intestine
Pepsin
Pancreatin
Amiloglucosidasa
2.5
6.5
30 min
2 h
(Carnachan
et al.
, 2012)
Stomach
Small intestine
Pepsin
Pancreatin
Bile salts
2.0
6.5
2 h
2 h
(Cilla
et al
., 2008, 2011)
Mastication
Na
2
HPO
4
, KH
2
PO
4
, NaCl
a
-amylase
Pepsin
Pancreatin
Bile salts
NaCl, KCl
6.75
1.2
6.0
10 min
2 h
1 h
(Gawlik-Dziki
et al
., 2009)
Stomach
Small intestine
Ascending colon
Transverse colon
Descending colon
Sigmoid colon
Faecal inoculum, nutrient medium
Faecal inoculum, nutrient medium
Faecal inoculum, nutrient medium
Faecal inoculum, nutrient medium
5.5
6.0
6.5
7.0
(Makivuokko
et al
., 2006;
Makelainen
et al
., 2007)
48 h
Stomach
Duodeno
Chlorhidric acid
Bile
Pancreatin
Sodium bicarbonate
Sodium bicarbonate
Sodium bicarbonate
Faecal samples
Nutrient medium
Dialysis membrane
4.5-1.7
6.5
1.5 h
1 day
(Minekus
et al
., 1995; Yoo &
Chen, 2006)
Jejunum
Ileum
Large intestine
6.8
7.2
5.8
3 d
Stomach
Small intestine
Transverse colon
Descending colon
Sigmoid colon
2.0-2.5
5.0-6.0
5.5-6.0
6.0-6.4
6.6-6.9
2 h
6 h
18 h
36 h
22 h
(Molly
et al
., 1993;
De Boever
et al
., 2000;
Possemiers
et al
., 2004)
Oxgall, pancreatine, NaHCO
3
Faecal sample, carbohydrates
Faecal sample, carbohydrates
Faecal sample, carbohydrates
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