Biology Reference
In-Depth Information
120.00
100.00
80.00
60.00
40.00
20.00
0.00
0
50
100 150 200
Concentration (ppm)
250
300
350
n
-Hexane
Aqueous
Ethyl acetate
Methanol
Fig. 15.3.
Scavenging of DPPH radicals by extracts from
C. talcana
.
Table 15.8.
Alkane composition (% total) of two populations of
Condalia microphylla
(collections 1 and 2)
compared with two populations of the Cordoba Area, Argentina
a
.
Compounds
Collection 1
Collection 2
Population 6
b
Population 14
c
Nonadecane (C-19)
4.0
4.8
4.9
3.2
Heinecosane (C-21)
2.2
2.0
2.1
2.0
Tricosane (C-23)
3.0
2.9
3.1
4.0
Pentacosane (C-25)
8.1
7.9
8.1
6.2
Heptacosane (C-27)
4.7
4.5
4.5
9.9
Octacosane (C-28)
3.8
3.8
5.5
6.3
Nonacosane (C-29)
31.2
29.0
26.0
30.0
Triacontane (C-30)
3.9
3.9
4.8
5.0
Hentriacontane (C-31)
33.7
32.7
34.3
12.4
Tritriacontane (C-33)
5.0
6.1
6.0
15.5
Unknown
0.4
2.4
0.7
5.5
a
For populations 6 and 14, please see Zygadlo
et al.
(1992).
b
C. microphylla
f. xanthocarpa (Cordoba area, Argentina).
c
C. microphylla
f. melanocarpa (Cordoba area, Argentina).
the yield of
Condalia
neo-triacontanes and
triacontanes (C-27, C-29, C-30, C-31 and
C-33), using conventional extraction proce-
dures (>35%), was comparable with litera-
ture data (Zygadlo
et al.
, 1992; Frontera
et
al.
, 2000). On the other hand,
n
-hexane
direct extraction was a little more efficient
in its isolation from the plant (data not
shown) than the conventional method
with methanol. Interestingly, the yield of
n-
alkanes of the Chilean collection (collec-
tion 1) was slightly higher than that obtained
for the Argentinian collection (collection 2).
This phenomenon could be explained by
the normal variation in the plant and by the
phytogeographic characteristics of the col-
lection sites, 'Montane grasslands and
shrublands' in Argentina and 'Mediterranean
forests woodlands and shrubs' in Chile,
with the latter more arid than the former.
The
n-
alkane composition determined in
this work is similar to the composition of
Condalia
complex in the
n-
hexane extracts
published by Zygadlo
et al.
(1992).
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