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CH 2 OH
O
CH 2 OH
O
O
+
HO
HO
HO
OH: H
NH 2
NH 2
n
Chitooligosaccharide
D-(+)-Glucosamine
NaCNBH 3
pH 6.2
CH 2 OH
O
O
HO
NH
n
CH 2 OH
OH
HO
HO
NH 2
Aminoglycosylated derivative
Fig. 14.4. Reductive alkylation of chitooligosaccharide to obtain the aminoglycosylated derivative.
to be about 700 kDa. The constituent mono-
saccharides were determined by means of
acid hydrolysis of the polymer and later
chromatographic analysis and spectroscopy
(Lillo et al ., 2007).
Huber and collaborators (1984) deter-
mined the composition of the major acid
EPS of 25 strains of Rhizobium japonicum .
Eight strains synthesized an acidic EPS con-
taining rhamnose and 4- O -methylglucuronic
acid and were closely related according to
DNA homology. The same strains also
expressed high levels of ex planta nitroge-
nase activity. Of the 25 strains, 16 produced
an acidic EPS containing glucose, mannose,
galacturonic acid and galactose, and were
also related by DNA homology. These
strains developed little or no nitrogenase
activity under the experimental conditions
employed.
CH 2 OH
O
HO
NH 2
O
n
Fig. 14.5. 1 4-2-amino-2-deoxy- a - d -galactan
(poly- a - d -galactosamine).
culture medium. The growth kinetics of
Paecilomyces sp. and the production of EPS
are shown in Fig. 14.6.
On the other hand, the exopolysaccha-
ride of Paecilomyces sp. was analysed by
gel permeation chromatography, which
revealed the existence of a homogeneous
EPS. The molecular weight was estimated
 
 
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