Alkylresorcinols: Chemical Properties, Methods of Analysis and Potential Uses in Food, Industry and Plant Protection - Natural Antioxidants and Biocides from Wild Medicinal Plants - page 155

or 5% phenyl-methylpolysiloxane (Suzuki
et al. , 1997). Several GC conditions have
been used with or without split injection.
In our laboratory, good resolution of ARs
without derivatization was obtained using
a capillary column Perkin-Elmer Elite-5MS
(5% phenyl-methylpolysiloxane, 30 m ×
0.25 mm i.d., 0.25 mm film thickness),

under the following conditions: 1 ml of the
sample disolved in ethyl acetate (50 ml/mg)
was injected to the column and a
temperature gradient from 170 to 320°C
(30 min) and 320°C (10 min). Helium was
used as carrier gas at a flow of 1.2 ml/min,
and split flow was adjusted to 80:1.
Injector temperature was 280°C (Fig. 10.5).

Abundance

28.59

1.5e+07

C21:0

1.45e+07
1.4e+07
1.35e+07
1.3e+07
1.25e+07
1.2e+07
1.15e+07

320°C (10 min)

170°C (0 min)

5°C / min

1.1e+07
1.05e+07
1e+07
9500000
9000000
8500000
8000000
7500000
7000000
6500000

C23:0

30.90

C19:0

25.96

6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000

C25:0

33.70

26.89

1500000
1000000
500000
0

25.81

C17:0

6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00

Time

26.00 28.00 30.00 32.00 34.00 36.00 38.00

Fig. 10.5. GC-MS chromatogram of the alkylresorcinols (ARs) extracted from wholewheat grains.
The ARs were injected without previous derivatization.