Biomedical Engineering Reference
In-Depth Information
fast-growing suspension cells in the BioWave, the AppliFlex, and the WUB.
However, up to now, wave-mixed bag bioreactors have not achieved the same
importance for the production of plant cell culture-derived products as they
have for animal-cell-based target molecules. The same is the case for microbial
products with pharmaceutical significance.
However, a recent modification of 2D bags by MayaBio has made it possible
to use the wave-mixed systems for every type of cell and organism; studies
reported by MayaBio show bacterial ODs of 70-80 in overnight cultures. The
MayaBioReactor introduces a proprietary sparger in Wave Bioreactor bags
that allows extensive aeration. Another major difference comes in the plat-
form, which is kept stationary and a pedal pushing up and down at one end
of the bag creates wave motions inside the bag, allowing mixing achieved by
using a rocking platform.
There is still interest in developing photo bioreactors, and Applikon has
recently made a disposable offering (Figure 5.10). A number of recent studies
have demonstrated that normal plants could be grown under light-emitting
diode light sources very efficiently. These solid-state lamps (SSLs) are tiny
semiconductor chips that generate light when powered. The elements that the
diode is made from determine the light spectrum it emits. These solid-state
devices have been improved over the years and now have greatly increased
light intensity and specific wavelengths. These developments have resulted
FIGURE 5.10
Applikon photo bioreactor.
