Biomedical Engineering Reference
In-Depth Information
FIGURE 5.9
CELL-tainer culture system.
fully automated SimCell MicroBioreactor System (with parallel disposable
cassettes and six microbioreactors per plate) ensures efficient process opti-
mizations for animal cell cultures, which can be transferred to stirred pro-
cesses with high reproducibility.
Wave-Mixed Bioreactors
These comprise a bag that consists of a multilayer film; ethylene vinyl acetate
(EVA) is the contact layer in most cases. The mixing takes place in the bag by
moving the platform sections. Oxygen is taken up from surface renewal of
waves formed, leaving a bubble-free surface.
A variety of designs, degree of bioinformatics, and sizes are available in
this category. Except for the WUB and CELL-tainer, the wave is caused by a
one-dimensional horizontal oscillation of the culture broth in the bag located
on a rocker unit. The intensity of the mass and energy transfer and, there-
fore, cell growth and product expression can be directly controlled through
wave generation and propagation. These features are adjustable by modify-
ing the rocking rate, the rocking angle, the filling level of the bag (up to 50%
maximum), and the aeration rate of the Wave Bioreactor, the BioWave, its suc-
cessor (the BIOSTAT CultiBag RM), the AppliFlex, the Tsunami Bioreactor,
and the CELL-tainer.
