Biomedical Engineering Reference
In-Depth Information
FIGURE 5.8
Celluxus bioreactor.
Cellexus Bioreactor
With exception of the WUB, the SBB, and the microbial versions of the XDR,
CELL-tainer and CellMaker, all disposable bioreactors have been developed
primarily for fed-batch operations with animal suspension cells (FigureĀ 5.8).
This kind of operation is most common in biomanufacturing. Anchorage-
dependent (adherent) cells are less widespread in today's processes; however,
disposable bioreactors such as AmProtein's CURRENT Perfusion Bioreactor
do allow the cultivation of adherent cells if they are grown on microcarriers.
Microcarriers also support the cell attachment to a 3D structure, enabling a
higher cell density and productivity, and culture conditions that are nearly
identical to an in vivo environment.
CELL-Tainer Cell Culture System
The fixed-bed bioreactors include the FibraStage (using FibraCel disks in
four disposable bottles per bioreactor system, with a maximum volume of
O.5 L CV per bottle) from New Brunswick Scientific and Artelis's fixed-
bed bioreactor (iCELLis bioreactor, with a maximum volume of 500 mL
per packed bed). Both bioreactors, which require microcarriers, were spe-
cifically designed for the production of cell-culture-based animal cells.
The FibraStage is kept in an incubator and is suitable for production at a
laboratory scale ( FigureĀ 5.9 ).
A novel small 3D bioreactor is Hamilton's BioLevitator operating with
modified, surface-aerated 50 mL plastic tubes, which oscillate vertically. The
 
Search WWH ::




Custom Search