Biomedical Engineering Reference
In-Depth Information
FIGURE 5.5
Cultibag bioreactor.
FIGURE 5.6
Applikon bioreactor.
branes wound around a tumbling stirrer, all features making it a simple
reactor to operate and test the expression of new cell lines (
Figure 5.7
).
Orbital shaker bioreactors comprise the third-largest category after the
wave and stirring types and promise high-throughput systems for scal-
ing up to pilot scale. Screening systems such as the M24 Microbioreactor
(Applikon, Pall Life Sciences), the BioLector (mp2-labs), and the Sensolux
(Sartorius-Stedim) are typically equipped with noninvasive single-use sen-
sors useful for PAT work. Sine orbital shaking was first applied to flasks
and plates, and these can still be upgraded to reactor level by connect-
ing them to a PreSens's Sensor Dish Reader (SDR) or a Shake Flask Reader
(SFR) using precalibrated sensor patches for pH and DO. The CultiFlask
50 disposable bioreactor and the Disposable Shaken Bioreactor System (a
cooperation between ExcellGene, Kiihner, and Sartorius-Stedim) and the
CURRENT Bioreactor (AmProteins) serve as midsize reactors, Zeta's bio-t
