Biomedical Engineering Reference
In-Depth Information
TABLE 1.1
Recombinant Production Engines
Host
Organism
Most Common
Applications
Advantages
Potential Challenges
Cell-free
Rapid expression
screening; toxic
proteins;
incorporation of
unnatural labels or
amino acids;
functional assays;
protein interactions
Rapid expression
directly from
plasmid; open
system: easily add
components to
enhance solubility
or functionality;
simple format;
scalable
Expression yields over
3 mg
Bacteria
Structural analysis;
antibody generation;
functional assays;
protein interactions
Scalable; low cost;
simple culture
conditions
Protein solubility;
minimal
posttranslational
modifications; may be
difficult to express
functional mammalian
proteins
Yeast
Structural analysis;
antibody generation;
functional assays;
protein interactions
Eukaryotic protein
processing;
scalable up to
fermentation
(g/L); simple
media
requirements
Fermentation required
for very high yield;
growth conditions
may require
optimization
Insect
Functional assays;
structural analysis;
antibody generation
Posttranslational
modifications;
similar to
mammalian
systems; greater
yield than
mammalian
systems
More demanding
culture conditions
Mammalian
Functional assays;
protein interactions;
antibody generation
Highest level of
correct
posttranslational
modifications;
highest probability
of obtaining fully
functional human
proteins
Multi-mg/L yields only
possible in suspension
culture; more
demanding culture
conditions
technique at milligram scales to industrial production at kilogram scales. The
era of biopharmaceuticals is manifested in the capability of producing large
quantities of biologics in stainless steel bioreactors. Today, those large-scale
stirred-tank bioreactors (usually >10,000 L in scale) represent modern mam-
malian cell culture technology, a major workhorse of the biopharmaceutical
 
Search WWH ::




Custom Search