Biomedical Engineering Reference
In-Depth Information
disposable-format expanded bed adsorption and high-capacity monolith
and membrane adsorbers to improve capacity during the capture.
MayaBio (
www.mayabio.com
) has recently developed a separative bioreac-
tor where the binding resin is added directly to the biroeactor contained in
a filter pouch with 30-micron-size screen; once the protein in the media is
absorbed onto resin or membrane, the bioreator is drained out. The drain is
closed, the resin/membrane is washed with buffers to remove debris, and
is finally washed with an eluting buffer to a volume that is generally less
than 5% of the original media volume. This process eliminates several steps:
bioseparation, ultrafiltration, and buffer exchange. If the resin/membrane
used to capture protein can be used in the column, then this approach elimi-
nates the lengthy procedure of column loading as well.
