Biomedical Engineering Reference
In-Depth Information
Top-plate port
Analyzer
TRACK
Off-line
Off-line filtration
Off-line dialysis
Sideport
FIGURE 7.1
Groton BioSystems Sampling Method.
forms of these are not yet available. Suppliers include TraceBiotech ( www.
trace.de ) and Groton ( www.grotonbiosystems.com ) (FigureĀ 7.1).
TRACE System
Where removing cells prove cumbersome, the samples may be treated to
stop their metabolic activity by freezing or using inactivation chemicals.
One way to solve the sampling problem is to use a presterilized sampling
container, including a needleless syringe that can be welded to the sampling
module of the bag bioreactor. A sample is pumped into the container, the
sampling containers can be removed, and the tube heat-sealed. Sartorius and
GE use this method. Other fully sampling systems involve connecting to a
bioreactor a presterilized Luer connection including a one-way valve to pre-
vent the sample from flowing back into the reactor. The sample is withdrawn
from the reactor by a syringe and directed through a sample line into a res-
ervoir. Cellexus Biosystems (Cambridgeshire, UK) and Millipore (Billerica,
MA) use this approach.
The Cellexus system is connected to the sample line, and there are up to six
sealed sample pouches. The sample from the reservoir can then be pushed
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