Biomedical Engineering Reference
In-Depth Information
51.
Biochem Biophys Res Commun
.
2006 Jun 30; 345(2):602-7. Epub
2006 Apr 19. Expression of a
human anti-rabies virus
monoclonal antibody in tobacco
cell culture. Girard LS, Fabis MJ,
Bastin M, Courtois D, Pétiard V,
Koprowski H. Thomas Jefferson
University, 1020 Locust Street,
Biotechnology Foundation,
Philadelphia, PA 19107.
A
Nicotiana tabacum
cv. Xanthi cell culture was
initiated from a transgenic plant expressing a human
antirabies virus monoclonal antibody. Within 3
months, plant cell suspension cultures were
established, and recombinant protein expression was
examined. The antibody was stably produced during
culture growth. ELISA, protein G purification,
Western blotting, and neutralization assay confirmed
that the antibody was fully processed, with
association of light and heavy chains, and that it was
able to bind and neutralize rabies virus.
Quantification of antibody production in plant cell
suspension culture revealed 30 microg/g of cell dry
weight for the highest-producing culture (0.5 mg/L),
3 times higher than from the original transgenic
plant. The same production level was observed 3
months after cell culture initiation. Plant cell
suspension cultures were successfully grown in a
new disposable plastic bioreactor, with a growth rate
and production level similar to that of cultures in
Erlenmeyer flasks.
52.
Biophys J
. 2006 May 15;
90(10):3813-22. Epub 2006 Feb
24. Soft trapping and
manipulation of cells using a
disposable nanoliter biochamber.
Diop M, Taylor R. Department of
Physics, The University of
Western Ontario, London,
Ontario, Canada.
Low-power continuous-wave laser radiation is used
to form a very stable microbubble at the end of a
specially etched and metalized optical fiber probe.
We demonstrate that the microbubble, which is
firmly attached to the fiber probe, can be used to
benignly trap and manipulate living swine sperm
cells as well as human embryonic kidney cells. The
lifetime of the microbubble has been prolonged and
the gaseous environment inside the bubble
controlled using micropipette gas injection. The
controlled fusion of two microbubbles is
demonstrated as a means of transferring
microparticles from one bubble to another. These
experiments lay the foundation for the use of the
microbubble as a mobile, nanoliter-volume
disposable biochamber for cellular studies.
