Biomedical Engineering Reference
In-Depth Information
46.
Biotechnol Bioeng
. 2007 Apr 1;
96(5):914-23. Two new
disposable bioreactors for plant
cell culture: the wave and
undertow bioreactor and the
slug bubble bioreactor. Terrier B,
Courtois D, Hénault N, Cuvier
A, Bastin M, Aknin A, Dubreuil
J, Pétiard V. Centre de Recherche
& Développement Nestlé-Tours,
101, Avenue Gustave Eiffel, BP
49716, 37097 Tours Cedex 2,
France.
benedicte.terrier@rdto.
The present article describes two novel flexible
plastic-based disposable bioreactors. The first one,
the wave and undertow (WU) bioreactor, is based on
the principle of a wave and undertow mechanism
that provides agitation while offering convenient
mixing and aeration to the plant cell culture
contained within the bioreactor. The second is a
high-aspect-ratio bubble column bioreactor, where
agitation and aeration are achieved through the
intermittent generation of large-diameter bubbles,
“Taylor-like” or “slug bubbles” (SB bioreactor). It
allows an easy volume increase from a few liters to
larger volumes up to several hundred liters with the
use of multiple units. The cultivation of tobacco and
soya cells producing isoflavones is described up to 70
and 100 L working volume for the SB Bubble Column
(SB) bioreactor and WU bioreactor, respectively. The
bioreactors are disposable and presterilized before
use, so cleaning, sterilization, and maintenance
operations are greatly reduced or eliminated. Both
bioreactors represent efficient and low-cost cell
culture systems, applicable to various cell cultures at
small and medium scale, complementary to
traditional stainless steel bioreactors.
47. J Biosci. 2006 Sep;31(3):363-8.
Aujeszky's disease virus
production in disposable
bioreactor. Slivac I, Srcek VG,
Radosevic K, Kmetic I, Kniewald
Z. Laboratory for Cell Culture
Technology and
Biotransformations, University
of Zagreb, 6 Pierotti St.,
HR-10000 Zagreb, Croatia.
A novel, disposable-bag bioreactor system that uses
wave action for mixing and transferring oxygen was
evaluated for BHK 21 C13 cell line growth and
Aujeszky's disease virus (ADV) production. Growth
kinetics of BHK 21 C13 cells in the Wave Bioreactor
during the 3-day period were determined. At the end
of the 3-day culture period and cell density of 1.82 ×
10(6) cells mL
−1
, the reactor was inoculated with 9
mL of gE- Bartha K-61 strain ADV suspension
(10(5.9) TCID50) with multiplicity of infection (MOI)
of 0.01. After a 144 h incubation period, 400 mL of
ADV harvest was obtained with a titer of 10(7.0)
TCID 50 mL
−1
, which corresponds to 40,000 doses of
vaccine against AD. In conclusion, the results
obtained with the Wave Bioreactor using BHK 21 C13
cells showed that this system can be considered
suitable for ADV or BHK 21 C13 cell biomass
production.
