Biomedical Engineering Reference
In-Depth Information
45. Biosens Bioelectron . 2007 Apr 15;
22(9-10):2071-8. Epub 2006 Oct
13. Design and development of a
highly stable hydrogen peroxide
biosensor on screen printed
carbon electrode based on
horseradish peroxidase bound
with gold nanoparticles in the
matrix of chitosan. Tangkuaram
T, Ponchio C, Kangkasomboon T,
Katikawong P, Veerasai W.
Department of Chemistry,
Faculty of Science, Mahidol
University, Bangkok 10400,
Thailand. pengtangkua@yahoo.
com
The design and development of a screen-printed
carbon electrode (SPCE) on a polyvinyl chloride
substrate as a disposable sensor is described. Six
configurations were designed on silk screen frames.
The SPCEs were printed with four inks: silver ink as
the conducting track, carbon ink as the working and
counter electrodes, silver/silver chloride ink as the
reference electrode, and insulating ink as the
insulator layer. Selection of the best configuration
was done by comparing slopes from the calibration
plots generated by the cyclic voltammograms at 10,
20, and 30 mM K(3)Fe(CN)(6) for each configuration.
The electrodes with similar configurations gave
similar slopes. The 5th configuration was the best
electrode that gave the highest slope. Modifying the
best SPCE configuration for use as a biosensor,
horseradish peroxidase (HRP) was selected as a
biomaterial bound with gold nanoparticles (AuNP)
in the matrix of chitosan (HRP/AuNP/CHIT).
Biosensors of HRP/SPCE, HRP/CHIT/SPCE and
HRP/AuNP/CHIT/SPCE were used in the
amperometric detection of H(2)O(2) in a solution of
0.1M citrate buffer, pH 6.5, by applying a potential of
−0.4 V at the working electrode. All the biosensors
showed an immediate response to H(2)O(2). The
effect of HRP/AuNP incorporated with CHIT (HRP/
AuNP/CHIT/SPCE) yielded the highest
performance. The amperometric response of HRP/
AuNP/CHIT/SPCE retained over 95% of the initial
current of the 1st day up to 30 days of storage at 4°C.
The biosensor showed a linear range of 0.01-11.3 mM
H(2)O(2), with a detection limit of 0.65 microM H(2)
O(2) (S/N=3). The low detection limit, long storage
life, and wide linear range of this biosensor make it
advantageous in many applications, including
bioreactors and biosensors.
Search WWH ::




Custom Search