Biomedical Engineering Reference
In-Depth Information
41.
Biotechnol Prog
. 2007 Jan-Feb;
23(1):46-51. Cell culture process
development: advances in
process engineering. Heath C,
Kiss R. Amgen, Seattle,
Washington 98119, and
Genentech, South San Francisco,
California 94080.
kiss.robert@
Representatives from the cell culture process
development community met on September 11 and
12, 2006 at the ACS National Meeting in San
Francisco to discuss “Cell Culture Process
Development: Advances in Process Engineering.”
This oral session was held as part of the Division of
Biochemical Technology (BIOT) program. The
presentations addressed the very small scale (less
than 1 mL) to the very large scale (20,000 L). The
topics covered included development of high-
throughput cell culture screening systems, modeling
and characterization of bioreactor environments from
mixing and shear perspectives at both small and
large scales, systematic approaches for improving
scale-up and scale-down activities, development of
disposable bioreactor technologies, and novel
perfusion culture approaches. All told, this well-
attended session resulted in a valuable exchange of
technical information and demonstrated a high level
of interest within the process development
community.
42.
Open Biomed Eng J
. 2007 Oct 29;
1:64-70. Cultivation and
differentiation of encapsulated
hMSC-TERT in a disposable
small-scale syringe-like fixed bed
reactor. Weber C, Pohl S, Pörtner
R, Wallrapp C, Kassem M, Geigle
P, Czermak P. Institute of
Biopharmaceutical Technology,
University of Applied Sciences
Giessen-Friedberg,
Giessen-Germany.
The use of commercially available plastic syringes is
introduced as disposable small-scale fixed-bed
bioreactors for the cultivation of implantable
therapeutic cell systems on the basis of an alginate-
encapsulated human mesenchymal stem cell line.
The system introduced is fitted with a noninvasive
oxygen sensor for the continuous monitoring of the
cultivation process. Fixed-bed bioreactors offer
advantages in comparison to other systems due to
their ease of automation and online monitoring
capability during the cultivation process. These
benefits combined with the advantage of single use
make the fixed-bed reactor an interesting option for
GMP processes. The cultivation of the encapsulated
cells in the fixed-bed bioreactor system offered
vitalities and adipogenic differentiation similar to
well-mixed suspension cultures.
