Biomedical Engineering Reference
In-Depth Information
35.
Biotechnol Prog
. 2007 Nov-Dec;
23(6):1340-6. Epub 2007 Oct 3.
Novel orbital shake bioreactors
for transient production of CHO
derived IgGs. Stettler M, Zhang
X, Hacker DL, De Jesus M,
Wurm FM. Laboratory of
Cellular Biotechnology, Faculty
of Life Sciences, Ecole
Polytechnique Fédérale de
Lausanne, CH-1015 Lausanne,
Switzerland.
Large-scale transient gene expression in mammalian
cells is being developed for the rapid production of
recombinant proteins for biochemical and preclinical
studies. Here, the scalability of transient production
of a recombinant human antibody in Chinese
hamster ovary (CHO) cells was demonstrated in
orbitally shaken disposable bioreactors at scales from
50 mL to 50 L. First, a small-scale multiparameter
approach was developed to optimize the
poly(ethylenimine)-mediated transfection in 50 mL
shake tubes. This study confirmed the benefit, both
in terms of extended cell culture viability and
increased product yield, of mild hypothermic
cultivation conditions for transient gene expression
in CHO cells. Second, the scalability of the process
was demonstrated in disposable shake bioreactors
having nominal volumes of 5, 20, and 50 L with final
antibody yields between 30 and 60 mg L(-1). Thus,
the combination of transient gene expression with
disposable shake bioreactors allows for rapid and
cost-effective production of recombinant proteins in
CHO cells.
36.
Biotechnol Lett
. 2008 Feb;
30(2):253-8. Epub 2007 Sep 22.
Bioreactor for solid-state
cultivation of Phlebiopsis
gigantea. Virtanen V, Nyyssölä
A, Vuolanto A, Leisola M,
Seiskari P. Laboratory of
Bioprocess Engineering, Helsinki
University of Technology, Espoo,
Finland.
veera.virtanen@kcl.i
Phlebiopsis gigantea
fungus used in biological control of
root rot is currently cultivated commercially in
disposable, sterilizable plastic bags. A novel-packed
bed bioreactor was designed for cultivating
P. gigantea
and compared to the plastic bag method
and to a tray bioreactor. The spore viability of
5.4 × 10
6
c.f.u./g obtained with the packed bed
bioreactor was of the same order of magnitude as the
viabilities obtained with the other cultivation
methods. Furthermore, the packed bed bioreactor
was less time- and space-consuming and easier to
operate than the tray bioreactor.
