Biomedical Engineering Reference
In-Depth Information
24. Adv Biochem Eng Biotechnol . 2010;
115:89-115. Disposable
bioreactors for plant
micropropagation and mass
plant cell culture. Ducos JP,
Terrier B, Courtois D. Nestlé
R&D Centre Tours, 101 Avenue
Gustave Eiffel, Notre Dame
D'Oé, BP 49716, 37097, Tours
Cedex 2, France.
Different types of bioreactors are used at Nestlé R&D
Centre - Tours for mass propagation of selected plant
varieties by somatic embryogenesis and for
large-scale culture of plants cells to produce
metabolites or recombinant proteins. Recent studies
have been directed to cut down the production costs
of these two processes by developing disposable cell
culture systems. Vegetative propagation of elite plant
varieties is achieved through somatic embryogenesis
in liquid medium. A pilot-scale process has recently
been set up for the industrial propagation of Coffea
canephora (Robusta coffee). The current production
capacity is 3.0 million embryos per year. The
pregermination of the embryos was previously
conducted by temporary immersion in liquid
medium in 10 L glass bioreactors. An improved
process has been developed using a 10 L disposable
bioreactor consisting of a bag containing a rigid
plastic box (“Box-in-Bag” bioreactor), ensuring,
among other advantages, a higher light
transmittance to the biomass due to its horizontal
design. For large-scale cell culture, two novel flexible
plastic-based disposable bioreactors have been
developed from 10 to 100 L working volumes,
validated with several plant species (“Wave and
Undertow” and “Slug Bubble” bioreactors). The
advantages and the limits of these new types of
bioreactor are discussed, based mainly on our own
experience on coffee somatic embryogenesis and
mass cell culture of soya and tobacco.
25. Adv Biochem Eng Biotechnol . 2010;
115:145-69. Sensors in disposable
bioreactors status and trends.
Glindkamp A, Riechers D,
Rehbock C, Hitzmann B, Scheper
T, Reardon KF. Institute for
Technical Chemistry, Leibniz
University Hannover, Callinstr. 3,
30167, Hannover, Germany,
glindkamp@iftc.uni-hannover.de .
For better control of productivity and product quality,
detailed monitoring of various parameters is
required. Since disposable bioreactors are becoming
more and more important for biotechnological
applications, adequate sensors for this type of reactor
are necessary. The required properties of sensors
used in disposable reactors differ from those of
sensors for multiuse reactors. For example, sensors
that are in direct contact with the medium must be
inexpensive, but do not need a long lifetime, since
they can be used only once. This chapter gives an
overview of the state of the art and future trends in
the field of sensors suited for use in disposable
bioreactors. The main focus here is on in situ sensors,
which can be based on optical, semiconductor, and
ultrasonic technologies, but current concepts for
disposable sampling units are also reviewed.
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