Biomedical Engineering Reference
In-Depth Information
2. Biotechnol Lett . 2011 Jan 26.
TubeSpin bioreactor 50 for the
high-density cultivation of Sf-9
insect cells in suspension. Xie Q,
Michel PO, Baldi L, Hacker DL,
Zhang X, Wurm FM. Laboratory
of Cellular Biotechnology,
Institute of Bioengineering, École
Polytechnique Fédérale de
Lausanne, CH J2 506, Station 6,
1015, Lausanne, Switzerland.
Here we present the TubeSpin bioreactor 50
(TubeSpins) as a simple and disposable culture
system for Sf-9 insect cells in suspension. Sf-9 cells
had substantially better growth in TubeSpins than in
spinner flasks. After inoculation with 10 6 cells/mL,
maximal cell densities of 16 × 10 6 and 6 × 10 6 cells/
mL were reached in TubeSpins and spinner flasks,
respectively. In addition, the cell viability in these
batch cultures remained above 90% for 10 days in
TubeSpins but only for 4 days in spinner flasks.
Inoculation at even higher cell densities reduced the
duration of the lag phase. After inoculation at 2.5 ×
10 6 cells/mL, the culture reached the maximum cell
density within 3 days instead of 7 days as observed
for inoculation with 10 6 cells/mL. Infection of Sf-9
cells in TubeSpins or spinner flasks with a
recombinant baculovirus coding for green
fluorescent protein (GFP) resulted in similar
GFP-specific fluorescence levels. TubeSpins are thus
an attractive option for the small-scale cultivation of
Sf-9 cells in suspension and for baculovirus-mediated
recombinant protein production.
3. Chimia (Aarau) . 2010; 64(11):819-
23. Innovative, nonstirred
bioreactors in scales from
milliliters up to 1000 L for
suspension cultures of cells
using disposable bags and
containers—a Swiss
contribution. Werner S, Eibl R,
Lettenbauer C, Röll M, Eibl D,
De Jesus M, Zhang X, Stettler M,
Tissot S, Bürki C, Broccard G,
Kühner M, Tanner R, Baldi L,
Hacker D, Wurm FM. Zürcher
Hochschule für Angewandte
Wissenschaften, Institut für
Biotechnologie, Wädenswil.
Innovative mixing principles in bioreactors, for
example, using the rocking of a platform to induce a
backwards and forwards “wave,” or using orbital
shaking to generate a wave that runs around in a
cylindrical container, have proved successful for the
suspension cultures of cells, especially when
combined with disposable materials. This article
presents an overview of the engineering
characteristics when these new principles are applied
in bioreactors, and case studies covering scales of
operation from milliliters to 1000 L.
4. Chimia (Aarau) . 2010; 64(11):803-7.
Process monitoring with
disposable chemical sensors fit in
the framework of process
analysis technology (PAT) for
innovative pharmaceutical
development and quality
assurance. Spichiger S, Spichiger-
Keller UE. C-CIT AG,
Einsiedlerstr. 29, Wadenswil.
The innovative principle of enzymatic sensors applied
to monitor the feeding process in disposable
bioreactors is described. Innovative is the type of
enzyme immobilized within the “paste” to monitor
l-glutamate. Innovative is the application of the
miniaturized disposable sensor developed at C-CIT
AG for continuous monitoring. The sensor allows the
amount of the digested nutrient to be estimated from
the amperometric signal. Innovative is the wireless
signal transduction between the sensor mounted to
the bioreactor and the signal receiver. An example of
a process control run is given and, also, the
biocompatibility and the specifications of the
biosensors. The comparison of results evaluated by
different analytical methods is discussed.
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