Synthesis and Validation of Cyanine-Based Dyes for DIGE - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 83

Fig. 6. Comparison of average ratios of individual protein measurements in samples labeled with NEW and OLD dyes dilutions
(see text for details). Spot numbers are shown at the bottom of the graph, while standardized log abundances for proteins
selected are shown on the y -axis.

Table 3
Comparison of average ratios for individual spots in samples
labeled with OLD and NEW dye dilutions

Deviation from mean average
ratio (%)

£1 1-5 5-10 10-15 ³15

Number of spots (total = 25) 5 11 5 3 1

were shown to be stable for years when stored as dry solids under
argon, the dyes are equally stable when stored in anhydrous DMF
in the argon atmosphere.