Analysis of Proteins Using DIGE and MALDI Mass Spectrometry - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 65

individual probabilities assigned to the peptide MS/MS spectra;
therefore, it is important for the overall identifi cation success
rate of protein identifi cation to allow, at the minimum, two
high-quality MS/MS unique peptide matches characterized
with a peptide probability score above 95%.

Acknowledgments

The research described here was possible thanks to fi nancial sup-
port from the Systems-Proteomics Center—University of North
Carolina at Chapel Hill. We would like to thank Dr. Roger Madison
(Duke University Medical Center) for helping with the acquisition
and analysis of human BDNF.
Disclaimer: The research described in this article was reviewed by
the National Health and Environmental Effects Research
Laboratory, United States Environmental Protection Agency, and
approved for publication. Approval does not signify that the con-
tents necessarily refl ect the views of the Agency, nor does mention
of trade names or commercial products constitute endorsement or
recommendation for use.

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