Biology Reference
In-Depth Information
Fold-changes of
protein abundance
DIGE coverage
of pathway
Catabolic network
COO
-
CO
∼
SCoA
CO
∼
SCoA
CO
∼
SCoA
CO
∼
SCoA
Toluene
vs
. Benzoate
HO
O
1000
COO
-
COO
-
COO
-
COO
-
COO
-
100
10
1
9
+2
/13
BssA-E
BbsEF
BbsG
BbsH
BbsCD
1
COO
-
CO
∼
SCoA
Ethylbenzene
vs
. Benzoate
HO
O
O
O
1000
100
10
+3
/11
2
3
EbdA-D
Ped
Apc1-5
Bal
10
COO
-
1
p
-Ethylphenol
vs
. Benzoate
HO
O
O
1000
?
100
10
5
+4
/8
5
4
PchCF
ChnA
EbA309
XccABC
OH
OH
OH
OH
1
AcsA?
CO
∼
SCoA
Phenol
vs
. Benzoate
O
1000
100
10
2
+2
/11
6
PpsABC
PpcA-D
∼
PHO
3
-
OH
O
OH
1
p
-Cresol
vs
. Benzoate
TioL
COO
-
CHO
CO
∼
SCoA
1000
2
+2
/3
7
8
100
EbA5380
EbA5381
HbcL-1
10
OH
OH
OH
OH
1
HcrCAB
Benzyl al
cohol
vs
. Benzoate
COO
-
CH
2
OH
CO
∼
SCoA
CO
∼
SCoA
CHO
1000
100
2/2
9
10
11
AdhB
Ald
10
1
PadE-I
COO
-
o
-Aminobe
nzoate
vs
. Benzoate
COO
-
CO
∼
SCoA
O
1000
NH
2
NH
2
100
HbrCABD
3/5
12
PadB-D
CO
∼
SCoA
HbcL?
10
1
COO
-
COO
-
Phenylalanine
vs
. Benzoate
COO
-
CHO
1000
NH
2
O
100
Pdh?
14
5
+3
/14
13
PadJ
Pat?
Pdc
EbA5004
10
EbA5005
EbA5007
1
m
-Hydroxybenzoate
vs
. Benzoate
COO
-
CO
∼
SCoA
CO
∼
SCoA
CO
∼
SCoA
CO
∼
SCoA
1000
HO
O
HbcL
HbrA-D
EbA720/22
EbA723/38
EbA720/2
2
100
6/9
15
10
OH
OH
O
O
O
1
Fig. 5. Metabolic network of
Aromatoleum aromaticum
EbN1 for anaerobic degradation of aromatic growth substrates.
Numbered compounds represent adaptation substrates underlying these comprehensive 2D DIGE analyses (
57
) . Compound
numbering:
1
, toluene;
2
, ethylbenzene;
3
, acetophenone;
4
,
p
-ethylphenol;
5
,
p
-hydroxyacetophenone;
6
, phenol;
7
,
p
-cresol;
8
,
p
-hydroxybenzoate;
9
, benzyl alcohol;
10
, benzaldehyde;
11
, benzoate;
12
,
o
-aminobenzoate;
13
, phenylala-
nine;
14
, phenylacetate;
15
,
m
-hydroxybenzoate. Enzymes are assigned to reactions as previously predicted (
25,
55-
57
) .
Benzoate-adapted cells (
boxed
) served as references state. Fold-changes of protein abundance are displayed for most
analyzed test states on the
left
panel, shaded proteins have not been predicted by original genome annotation to be
involved in substrate metabolism. DIGE coverage of pathways (X + Y/Z) compares DIGE-identifi ed proteins (X) vs. predicted
proteins (Z), considering also additional co-regulated proteins (Y).
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