An Overview of 2D DIGE Analysis of Marine (Environmental) Bacteria - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 364

c

a b

Glucose
N -Acetylglucosamine

Maltose

CH 2 O~P

-Acetyl-
glucosamine

N

3330-1

9498

O

H OH 3330-2

Lactose

H

Eno

OH H

9527

Melibiose Xylose

Gap

HO

H

NH

H

Ribose

Raffinose

OC-CH 3

3342

N -Acetylglucosamine

-6-phosphate

Deacetylase?

CH 2 O~P

O

OH

H

H 5240

OH H

HO

H

NH 2

H

Glucosamine-6-phosphate

Glucosamine-6-P-isomerase
NagB

sugar-
epimerase

Deaminase
Isomerase

Myo-Inositol-2-DH

Dehydrogenase

CH 2 O~P

O 3330 3342

OH

H

H

OH H

HO

H

H

H Endo-1,4- β -xylanase Myo-Inositol-2-DH

Fructose-6-phosphate

5240 9527

Fig. 4. Carbohydrate metabolism in Rhodopirellula baltica SH1. ( a ) Carbohydrates are channeled via peripheral pathways into central metabolism; ( b ) the reaction sequence for
N -acetylglucosamine degradation is unclear at present, since a known deacetylase for initial acetyl-group removal is not encoded in the genome. ( c ) However, 2D DIGE analysis
revealed the specifi c formation of proteins of unknown function that could possibly be involved in the peripheral degradation of N -acetylglucosamine. Eno (enolase) and Gap
(glyceraldehyde-3-phosphate dehydrogenase) of central glycolysis are not regulated. Upregulated proteins are marked in red. Coloring of genes: gray , predicted function; hatched,
conserved hypothetical; white , unknown function.