Application of DIGE and Mass Spectrometry in the Study of Type 2 Diabetes Mellitus Mouse Models - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 316

Table 3
(continued)

Protein name (from
UniProtKB) Spot Prot_desc Prot_score Prot_mass Prot_matches Prot_cover Prot_pi Fold change a p -value a

481E P11679
Q63836
Q91XD4
Q61035
P07724

1,118
884
179
114
124

54.531
53.147
59.529
57.893
70.700

30
32
3
2
2

47.3
45.1
6.5
4.9
5.75

5.7
5.78
5.79
5.68
4

Keratin, type II
cytoskeletal 8
Selenium-binding protein 2
Formimidoyltransferase-
cyclodeaminase
Histidyl-tRNA synthetase,
cytoplasmic
serum albumin

2 NZO up 4.51E−13

484 Q63836

P17563
Q91XD4
P11679

1,667
1,290
657
236

53.147
53.051
59.529
54.531

113
102
14
6

71.4
55.7
29.2
11.2

5.78
5.87
5.79
5.7

Selenium-binding protein 2
Selenium-binding protein 1
Formimidoyltransferase-
cyclodeaminase
Keratin, type II
cytoskeletal 8

5.2 NZO up 1.11E−15

651 Q9Z0S1

P21278

541
95

33.517
42.283

47
3

35.7
8.4

5.54
5.7

3¢(2¢),5¢-bisphosphate
nucleotidase 1
Guanine nucleotide-binding
protein subunit alpha-11

2.9 NZO down 2.24E−08

654 Q9Z0S1 489 33.517 45 28.2 5.54 3¢(2¢),5¢-bisphosphate

nucleotidase 1

5.5 NZO up 3.16E−10

864 P16015 542 29.633 33 51.2 6.89 Carbonic anhydrase 3 2 NZO down 1.82E−12

865 P16015 241 29.633 10 20 6.89 Carbonic anhydrase 3 2 NZO down 3.30E−10

All identifi cations have at least two unique peptide matches in Mascot. Human and associated mouse keratins are excluded
a These values were obtained from Progenesis SameSpots; proteins are either signifi cantly up- or down-regulated for the genotype New Zealand Obese (NZO)
in comparison to the genotype Swiss Jim Lambert (SJL)