Application of DIGE and Mass Spectrometry in the Study of Type 2 Diabetes Mellitus Mouse Models - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 315

Table 3
Partial Mascot results list of signifi cant protein identifi cations for the differentially
expressed proteins in T2DM mouse models

Protein name (from
UniProtKB) Spot Prot_desc Prot_score Prot_mass Prot_matches Prot_cover Prot_pi Fold change a p -value a

359 Q8VC12 241 75.227 24 7.4 7.27 Urocanate hydratase 2.4 NZO down 3.87E−06

420 P32020

Q8CHT0
P24270
Q99L88
Q03265
P24549

605
479
467
135
114
89

59.715
62.228
60.013
58.444
59.830
55.060

23
11
13
4
2
2

30.5
17.1
22.6
9.1
4
5.6

7.16
8.58
7.72
8.51
9.22
7.92

Non-specifi c lipid-transfer
protein
Delta-1-pyrroline-5-
carboxylate
dehydrogenase
Catalase
Beta-1-syntrophin
ATP synthase subunit
alpha, mitochondrial
Retinal dehydrogenase 1

2.9 NZO down 2.16E−08

481 P11679

Q63836
Q91XD4
Q61035

1,648
925
347
102

54.531
53.147
59.529
57.893

194
118
11
3

54.3
36.7
15
5

5.7
5.78
5.79
5.68

Keratin, type II
cytoskeletal 8
Selenium-binding protein 2
Formimidoyltransferase-
cyclodeaminase
Histidyl-tRNA synthetase,
cytoplasmic

2 NZO up 4.51E−13

481W Q63836
P30416
P11679
Q91XD4

344
188
148
75

53.147
51.939
54.531
59.529

13
3
4
3

18.6
9.2
8.6
4.6

5.78
5.54
5.7
5.79

Selenium-binding protein 2
Peptidyl-prolyl cis-trans
isomerase
Keratin, type II
cytoskeletal 8
Formimidoyltransferase-
cyclodeaminase

2 NZO up 4.51E−13

(continued)