The Basics of 2D DIGE - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 18

performed. If the gels need to be stored prior to scanning,
then they can be kept under SDS running buffer in a light-
tight container at 4°C. It is recommended to allow the gels to
warm up to room temperature before imaging, as fl uorescent
intensity is temperature dependent. The ethanol used should
be free of hexanes or other nonalcohol organic solvent impuri-
ties that can contribute to background fl uorescence.

Acknowledgments

Many thanks to Rita Marouga, Viola Ruddat, and Chris Rozanas
(GE Healthcare) for their critical review and feedback.

Trademarks
CyDye™, Cy™, and Deep Purple™ are trademarks of GE Healthcare
Ltd., a General Electric company.
Coomassie ® is a registered trademark of ICI plc.
SYPRO ® is registered trademark of Molecular Probes, Inc.
Sigma-Aldrich ® is a registered trademark of Sigma Chemical Co.
2D Fluorescence Difference Gel Electrophoresis (Ettan DIGE)
technology is covered by US Patent Numbers 6,043,025,
6,127,134, 6,426,190 and foreign equivalents and exclusively
licensed from Carnegie Mellon University by GE Healthcare, Ltd.,
a General Electric company.

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