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the next-generation fl uorescence imager that has the requisite
dynamic range to detect proteins over a million-fold concentration
range. Once this dynamic range goal is achieved, the next goal will
be to isolate suffi cient quantities of protein from these very rare
protein spots to be identifi ed by mass spectrometry. I am confi dent
that these methods are within our reach. Thus, I feel that the future
for DIGE is very bright (please forgive the pun).
Acknowledgments
The development of DIGE would not have been possible without
the efforts and dedication of my students and staff (Mustafa Ünlü,
Liz Morgan, Chris Lacenere, Surya Viswanathan, Lei Gong,
Mamta Puri, Anupam Goyal, and Susan Down).
References
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2. Helling, S., Schmitt, E., Joppich, C.,
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5. Han, M. J., Herlyn, M., Fisher, A. B., and
Speicher, D. W. (2008) Microscale solution
IEF combined with 2-D DIGE substantially
enhances analysis depth of complex proteomes
such as mammalian cell and tissue extracts,
Electrophoresis
29
, 695-705.
6. Sitek, B., Sipos, B., Pfeiffer, K., Grzendowski,
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