Structural Deterioration in Produce: Phospholipase D, Membrane Deterioration, and Senescence - Postharvest Biology and Technology of Fruits, Vegetables, and Flowers - page 215

Table 9.3 Chemical and physical parameters of PLD alpha proteins encoded by genes in tomato (LePLD α 1,
LePLD α 2, and LePLD α 3), strawberry (FaPLD α 1), honeydew melon (CmPLD α 1 and CmPLD α 2), and
cucumber (CsPLD α 1)

Amino MM ASP + ARG + Acidic/ Theoretical Grand average

acids (kDa) GLU LYS basic pI hydropathicity

LePLD α 1 809 92.2 121 89 A 5.39 − 0 . 447

LePLD α 2 807 92.0 112 86 A 5.59 − 0 . 349

A −

LePLD α 3 806 92.7 105 94 6.29 − 0 . 378

α − .

FaPLD 1 810 91.4 114 91 A 5.76 0 395

α − .

CmPLD 1 808 92.0 122 92 A 5.37 0 483

A −

CmPLD α 2 807 92.1 109 97 6.20 − 0 . 398

CsPLD α 1 808 91.8 122 92 A 5.37 − 0 . 472

Parameters shown in columns 1-7 from left to right include total encoded amino acids, molecular mass (MM),
sum of aspartic plus glutamic acid residues (ASP + GLU), sum of arginine plus lysine residues (ARG + LYS),
acidic or basic polypeptide (A, acidic; A − , weakly acidic), theoretical isoelectric point (pI), and grand average
hydropathicity. PLD alpha protein accession numbers are shown in Table 9.2.

phospholipase D alpha 2 (CmPLDa2) [Cucumis melo var. inodorus]
phospholipase D alpha 2 (LePLDa2) [Lycopersicon esculentum]
phospholipase D alpha 3 (LePLDa3) [Lycopersicon esculentum]
phospholipase D1 [Craterostigma plantagineum]
phospholipase D2 [Craterostigma plantagineum]
phospholipase D alpha [Cynara cardunculus]
phospholipase D alpha 1 (PLD alpha 1) [Nicotiana tabacum]
phospholipase D alpha 1 (LePLDa1) [Lycopersicon esculentum]
phospholipase D alpha 1 (PLD alpha 1) [Pimpinella brachycarpa]
phospholipase D alpha [Vitis vinifera]
phospholipase D alpha 2 [Arachis hypogaea]
phospholipase D alpha 1 [Medicago truncatula]
phospholipase D alpha 2 [Medicago truncatula]
phospholipase D alpha 1 (CmPLDa1) [Cucumis melo var. inodorus]
phospholipase D [Cucumis sativus]
phospholipase D alpha [Gossypium hirsutum]
phospholipase D alpha 1 (PLD alpha 1) [Vigna unguiculata]
phospholipase D alpha 2 (PLD 2) [Brassica oleracea]
phospholipase D alpha 1 (PLD 1) [Brassica oleracea]

phospholipase D alpha 1 [Arabidopsis thaliana]

phospholipase D alpha [Arabidopsis thaliana]

phospholipase D Alpha 2 [Arabidopsis thaliana]

phospholipase D alpha 1 precursor (PLD 1) [Ricinus communis]

phospholipase D alpha [Fragaria × ananassa]

phospholipase D2 [Papaver somniferum]

phospholipase D1 [Papaver somniferum]

Fig. 9.11 Phylogenetic tree showing the relatedness of plant PLD alphas from 17 dicot species based on alignment
of their complete deduced amino acid sequences ranging from 806 to 813 residues. Accessions with arrowheads
on the right indicate the seven PLD alpha genes currently being investigated in relation to ripening and senescence
of tomato ( Lycopersicon esculentum —light gray), honeydew melon ( C . melo —medium gray), and strawberry
( F . ananassa —dark gray) fruits. The other two highlighted accessions indicate PLD alphas cloned from tissues

of grape berry ( V . vinifera —dark gray) and cucumber fruit ( C . sativus —light gray).

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