Programmed Cell Death during Plant Senescence - Postharvest Biology and Technology of Fruits, Vegetables, and Flowers - page 110

(a)
(b)
(c)
Fig. 5.4 Two-dimensional polyacrylamide gel electrophoresis analysis of total cellular protein from control cell
cultures and cell cultures undergoing PCD. Spots (1-12) identified as being unique to and/or increased in relative
abundance in both heat-treated and senescing samples undergoing PCD were excised, digested with trypsin, and
analyzed by MS/MS. Each gel result was repeated in triplicate: (a) untreated, 6-day-old cultures; (b) 6-day-old
cultures incubated for 10 min at 55 ◦ C and sampled immediately thereafter; (c) senescing, 13- to 14-day-old cultures
(Swidzinski et al., 2004).