Programmed Cell Death during Plant Senescence - Postharvest Biology and Technology of Fruits, Vegetables, and Flowers - page 107

work with the diverse species currently being studied to appreciate the range of networks
employed.
Another important question is whether the same signal differentially regulates PCD in
floral organs. In some cases the answer is yes: for example, in tobacco, ethylene regulates
petal senescence (Rieu et al., 2003); however, at the same time ovary tissues continue to
develop. So how is a primary signal such as ethylene transduced to ensure the coordinated
life and death of different floral organs? Presumably, this is through differential signal
translocation or differential signal perception. Petal margins often degenerate before the
center and cross sections of developing petals reveal that while the epidermal cells are still
functional, mesophyll cells have largely degenerated even before the flower is fully open
(Wagstaf et al., 2003). So, is there a gradient of a diffusible signal, or of receptors or other
intracellular mediators of the cell death signal? In some cases, this signaling differential is
very distinct: in the Arabidopsis gfa2 mutant, synergids fail to undergo PCD, but antipodal
PCD is not affected (Christensen et al., 2002). Ethylene is not the only PGR-stimulating
PCD in floral organs: some links to other PGRs are reviewed in Wu and Cheung (2000).
Mutation of gibberellic acid biosynthetic genes anther ear 1 and dwarf results in failure to
abort stamens on maize female flowers. Mutation of a gene associated with brassinosteroids
(TS) (“tasselseed”) results in feminization of male flowers, and application of jasmonate
(JA) enhances petal senescence in some species (Porat and Halevy, 1993), although this
effect may be indirect through ethylene signaling. Elevating cytokinin levels in petunia
delayed flower senescence; however, this may also be indirect through changes in sugar
transport (Lara et al., 2004). So are all these PGRs involved in floral PCD in all species?
Or are there important quantitative or even qualitative species-specific differences in their
effects? Perhaps metabolomic approaches to measure endogenous levels of PGRs, coupled
with a more extensive use of mutants, may begin to address these questions.
5.14 PCD mechanism in floral organs
van Doorn and Woltering (2005) have recently categorized plant PCD into three types:
apoptotic, autophagic, and neither apoptotic nor autophagic. In animal cells, four types of
apoptosis have been described (Orrenius et al., 2003), three of which involve cytochrome C
release from the mitochondrion controlled by a family of proteins (Bcl-2) that interact with
the mitochondrial membrane to facilitate or inhibit this process. Cytochrome C then acti-
vates a family of cysteine aspartate-specific proteases (caspases), which both regulate and
affect PCD. Apoptotic PCD in animals is characterized by cytological features, including
chromatin and nuclear condensation and marginalization followed by DNA fragmentation
into nucleosomal units known as DNA laddering, nuclear blebbing, and formation of mem-
brane inclusions known as apoptotic bodies (Cohen, 1993). The apoptotic bodies are then
engulfed by neighboring living cells. In the tapetum and pollen tubes, there is compelling
evidence to support an important role for the mitochondrion and involvement of caspases.
This suggests a mechanism similar to animal apoptosis, although caution must be exercised
in drawing too close a parallel, as engulfment of cellular remains by other cells does not
occur in plants (van Doorn and Woltering, 2005). Following its nutritive role during pollen
development, the tapetum degenerates. This is characterized by chromatin condensation in
Lobivia rauschii and Tillandsia albida (Papini et al., 1999), and by DNA fragmentation in
barley anthers (Wang et al., 1999).