Programmed Cell Death during Plant Senescence - Postharvest Biology and Technology of Fruits, Vegetables, and Flowers - page 93

(a) (a ′ )

(b) (b ′ )

(c) (d)

Fig. 5.3 TUNEL reaction. Whole mounts of petal pieces collected 2 days after anthesis were assayed with the
TUNEL reaction. Increase in DNA ends was highlighted by addition of nucleotide analogs by using TdT, and
the incorporated analogs decorated with the use of alkaline phosphatase linked to antibodies against the nucleotide
analogs and NBT-BCIP substrates. (a) Petals treated with STS at stage d-1. (b-d) Nontreated petals. (a ,b ) Same
as (a) and (b) but after TUNEL staining petals were incubated with Acridine Orange to visualize the nuclei (Orzaez
and Granell, 1997a).

93