Agriculture Reference
In-Depth Information
presence/absence of a given protein but also the
potential post-translational modifications (e.g., phos-
phorylations, glycosylations or prenylations) and also
have the potential to assess protein-protein interac-
tions. Available platforms include the traditional 2D gel
electrophoresis - combined or not with fluorescent dyes,
as in the difference in gel electrophoresis (DIGE) tech-
nique - which is useful for protein fingerprinting when
coupled to mass spectrometry (MS) for protein
identification. The shotgun proteomics approach is
based on nano-liquid chromatography (nanoLC) sepa-
ration of matrices and MS detection; it offers a deeper
and less biased coverage of the proteome including low
abundant proteins (Zhao
et al.,
2013). Future chal-
lenges in proteomics are the development of new
analytical techniques and workflows to overcome the
lack of reproducibility, and the implementation of new
features for data export and comparison in databases
(Kosva
et al.,
2013). Several researchers have reported
that proteins respond to salt stress differently in
different plants including leguminous crops (Hakeem
et al.,
2012a; Komatsu & Hossain 2013; Kosva
et al.,
2013; Zhao
et al.,
2013) (Table 2.3).
Kav
et al.
(2004) studied the proteome-level changes
in the roots of
Pisum sativum
in response to salinity. This
study identified 35 protein spots that exhibited significant
changes in abundance due to NaCl treatment. These pro-
teins were identified as pathogenesis-related (PR) 10
proteins, antioxidant enzymes such as superoxide dis-
mutase (SOD) as well as nucleoside diphosphate kinase
(NDPK). The study suggested the possible existence of a
novel signal transduction pathway with a potentially
important role in abiotic stress response.
Jain
et al.
(2006) studied the proteome of a salinity-
tolerant
Arachis hypogea
L. callus cell line in comparison
with sensitive counterparts. Several low-molecular-
weight proteins were identified or significantly
upregulated in the tolerant line, and tandem mass spec-
trometry analysis revealed the presence of PR10 proteins.
The study recognized the role of the differentially
Table 2.3
Summary of proteomic publications in leguminous crops.
S. No.
Species
Variety
Tissue
Salt treatment
Proteomic
approach
IDs
Unique
proteins
References
1
Arachis hypogea
L.
JL-24
Callus cell
200 mM, 12 d
2DE/ESI-LC-MS-MS
25
6
Jain
et al.,
2006
2
Glycine max
L.
Enrei
Hypocotyls,
root
100 mM, 16 d
2DE/
ESI-QTOF-MS-MS
7
7
Aghaei
et al.,
2009
3
Enrei
Hypocotyls,
root, leaves
40 mM, 16 d
2DE/MALDI-TOF-MS
38
31
Sobhanian
et al.,
2010b
4
LEE 68 (ST)
N2899 (ST)
Germinating
seeds
100 mM until
radicle
protrusion from
the seed coat
2DE/MALDI-TOF-MS
18
18
Xu
et al.,
2011
5
Pusa-24 (SS)
Pusa-37 (ST)
Seedling
25, 50, 75,
100, 125 and
150 mM, 10 d
2DE/
MALDI-TOF-MS
173
40
Hakeem et al 2012
6
Jackson (SS)
Lee 68 (ST)
Leaf
150 mM, 1, 12,
72, 144 h
2DE/
MALDI-TOF-TOF-MS
91
78
Ma
et al.,
2012
7
Lathyrus sativus
L.
LP 24
Leaf
500 mM, 12 h,
24 h, 3 d, 6 d,
12 d
2DE/ESI-LC-MS/MS
44
30
Chattopadhyay
et al.,
2011
8
Lupine luteus
L.
Mister
Mitochondria
250, 500 mM,
12 h
2DE/ESI-LC-MS/MS
21
21
Wojtyla
et al.,
2013
9
Pisum sativum
L.
Cutlans
Root
75, 150 mM, 7
d; 75, 150 mM,
42 d
2DE/
ESI-QTOF-MS-MS
35
24
Nat
et al.,
2004
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