Biology Reference
In-Depth Information
FIGURE 18.3
Molecular phylogenetic analysis of three types of
wsp
sequences identiÝed from a laboratory
strain and six Japanese populations of
sequences were analyzed together with the
sequences investigated by Zhou et al. (1998). A total of 523 unambiguously aligned nucleotide sites were
subjected to the analysis. A neighbor-joining phylogeny is shown. On the right side are shown supergroups
(A and B) and groups (Mel-Pip) of
C. chinensis
. The
wsp
designated by Werren et al. (1995b) and Zhou et al. (1998).
The bootstrap values in percentage obtained with 1,000 resamplings are shown at the nodes, though values
smaller than 50% are not shown. The numbers in brackets are accession numbers. [From Kondo, N., Ijichi,
N., Shimada, M., and Fukatsu, T. (2002a).
Wolbachia
Mol. Ecol.
11: 167Ï180. With permission]
PREVAILING TRIPLE INFECTION IN NATURAL POPULATIONS
OF
C. CHINENSIS
Infection frequencies with wBruCon, wBruOri, and wBruAus in Japanese populations of
C. chin-
ensis
were examined by speciÝc PCR detection. Of 410 insects from nine Japanese local popula-
tions, wBruCon, wBruOri, and wBruAus were detected in 410 (100%), 395 (96.3%), and 398
(97.1%), respectively.
With the three
strains, there are eight possible infection states: a triple infection
(infection state: COA), three double infections (CO, CA, and OA), three single infections (C,
O, and A), and no infection. Among these infection states, triple infection (93.7%) was highly
Wolbachia
