Biology Reference
In-Depth Information
TABLE 6.1
Concentrations of Synthetic Cecropin A Lethal for
Escherichia coli
, the
Rhodococcus rhodnii
Symbiont, and
Three Strains of
Trypanosoma cruzi
Strain'
Concentration
E. coli
23
n
M
a
DH5
b
MCR
R. rhodnii
500
n
M
a
ATCC 35071
T. cruzi
240
n
M
b
Gainesville
DM28
140
n
M
b
ÑYÒ
150
n
M
b
Note:
The DM28 strain used in these
in vivo
experiments is a common human
Chagas pathogen in Brazil.
a
Values for
E. coli
and
R. rhodnii
are minimum bactericidal concentrations (MBC).
b
(epimastigote forms derived from cell culture) are
concentrations needed to kill 100% of parasites at 24 h of incubation (LC).
The values for
T. cruzi
Source:
Data from Durvasula, R.V., Gumbs, A., Panackal, A., Kruglov, O., Aksoy,
S., MerriÝeld, R.B., Richards, F.F., and Beard, C.B. (1997).
Proc. Natl. Acad. Sci.
U.S.A.
94:
3274Ï3278.
SspI
HindIII
XmnI
mat CecA
SalI
ScaI
Amp
R
Thio
R
promoter
XbaI
pRr Thio Cec
7.95 kb
BamHI
Thio
R
ori
Thio
R
promoter
ori
Rh
HaeII
BamHI
SmaI
KpaI
EcoRI
EcoRI
FIGURE 6.2
The shuttle plasmid pRrThioCec. [Adapted from Durvasula, R.V., Gumbs, A., Panackal, A.,
Kruglov, O., Aksoy, S., MerriÝeld, R.B., Richards, F.F., and Beard, C.B. (1997).
Proc. Natl. Acad. Sci. U.S.A.
94:
3274Ï3278.]
