Biomedical Engineering Reference
In-Depth Information
Figure 7.42
Kinetics of hybridization of DNA segments on a solid flat surface functionalized with
complementary DNAs. The dots correspond to the experimental results and the continuous line to
the calculation.
7.4.2.3
Advection-Diffusion and Biochemical Reactions
Introduction
Consider the case of a buffer fluid flowing through a microchamber comprising
one or more reactive surfaces (Figures 7.43 and 7.44). Usually such experimental
devices are used to find the kinetic constants
k
on
and
k
off
for a given sequence of
DNA.
Governing Equations
The advection-diffusion equation for concentration in the fluid volume is
∂
∂
c
=
D c
D - Ñ
v c
(7.85)
t
Figure 7.43
Schematic view of DNA hybridization in a microchannel (side view).
