Biomedical Engineering Reference
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Figure 7.20  BAEE (Benzoyl-Arginyl-Ethyl-Esther) reacting with trypsine in a beaker. (a) Measure-
ments of absorbance of light at 500 nm. (b) Experimental kinetics curves (concentration of Benzoyl
versus time). From top to bottom: initial concentrations of substrate 1, 0.7 and 0.4 mM.
Michaelis-Menten model and the piecewise analytical model from preceding
section have been used to interpret the experimental data (Figure 7.21). The piece-
wise analytical model fits better with the experimental data. However, as we have
mentioned earlier, it requires more physical parameters than the Michaelis-Menten
model, which remains a good trade off between simplicity and precision.
Figure 7.21  Comparison of reaction kinetics between experiments (dots), Michaelis-Menten model
(dotted line) and piecewise analytical model (continuous line).
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