Biomedical Engineering Reference
In-Depth Information
23k PRL
16k PRL
Luc(Control)
1
2
3
4
5
5
6
7
8
9
10
10
11
12
13
14
15
15
16
probe index
Fig. 5. Prolactin gene prole in probe level expression. The gure is the comparisons
of prolactin gene's probe expression among the 23K PRL, 16 PRL, and the control
groups. In this gure, a solid curve (-o-) represents probe level expression for the 23K
PRL, a dashed curve ( ) is for the 16 PRL, and a dotted curve (-+-) is for the
control group. Most probe expressions were dierential in the 23k PRL arrays, but only
about half of the probes showed expression changes in the 16k PRL arrays. The results
indicate homogenous dierential probe expressions occurred in the 23k PRL group, but
an interaction eect (expression dierences depend on probes) occurred in the 16k PRL
group.
biological functions. Here we used the comparison of 16 PRL versus the
control groups for illustration. Figure 6.1 is the main output of analysis
results. It shows the number of regulated genes (28 down-regulated and
35 up-regulated) in the rst table of the gure. The numbers of biological
functions related to gene expression change ranged from 8 to 60 in the sec-
ond table. For example, there were 42 annotations of GO biological process,
and 8 pathways involved in the down-regulated genes. A click of the down-
regulated cell in the table of total number of selected genes leads to the
Figure 6.2, where the 28 down-regulated genes are listed with gene name,
fold change, and gene description. The table allows investigators to rene
gene selections based on various criteria, such as fold change. Since there
may be multiple probe sets listed with the same gene names, the table was
sorted by gene name to provide a descriptive assessment of likelihood of
true dierential expression. If a gene name appears many times, the chance
Search WWH ::




Custom Search