Biology Reference
In-Depth Information
TABLE I
P
ROTEINS
I
NVOLVED IN
DNA M
ISMATCH
R
EPAIR
C
ORRECTION
g
-Proteobacteria (
E. coli
)
Other eubacteria (
B. subtilis
)
Eukaryotes (Humans)
Function
MutS
MutS
MutS
a
(MSH2-MSH6)
Recognition of base mismatches and 1-2 base indels
MutS
b
(MSH2-MSH3)
Recognition of
2 base indels
MutL
MutL*
MutL
a
(MLH1-PMS2*)
Molecular matchmaker in MMR (*endonuclease)
MutL
b
(MLH1-PMS1)
?
MutL
g
(MLH1-MLH3*)
Molecular matchmaker in meiosis (*endonuclease)
MutH
?
?
Nicks hemimethylated DNA (strand discrimination)
5
0
!
3
0
exonuclease
RecJ, ExoVII
RecJ, ExoVII
Exo1
ExoVII, ExoI, ExoX
-
-
3
0
!
5
0
exonuclease
Helicase II (UvrD)
Helicase II (UvrD)
-
3
0
!
5
0
helicase
Polymerase III
Polymerase III
Polymerase
d
DNA resynthesis
b
b
PCNA
Processivity subunit (sliding clamp)
g
-Complex
g
-Complex
RFC
Clamp loader complex
SSB
SSB
RPA
Protects ssDNA during excision and resynthesis
Ligase A
Ligase
Ligase 1
Seals nick after DNA resynthesis
