Biology Reference
In-Depth Information
TABLE III
S
UBUNIT
C
OMPOSITION OF
INO80
Yeast INO80
Human INO80
Ino80
hINO80
Rvb1
RUVBL1/Tip49a
Rvb2
RUVBL2/Tip49b
Arp4
BAF53a
Arp5
hArp5
Arp8
hArp8
Ies2
hIes2/PAPA-1
Ies6
hIes6/C18orf37
Act1
Taf14/Anc1
Ies1
Ies3
Ies4
Ies5
Nhp10
FLJ20309/INO80D
NFRKB
MCRS1
Amida
YY1
CCDC95/FLJ90652/INO80E
Uch37
Catalytic subunits are shown in bold. INO80 complexes from yeast and humans possess a core of conserved
subunits and additional yeast- or metazoan-specific subunits. The metazoan-specific subunits associate with the
N terminus of hINO80, while the conserved core subunits bind to central and C-terminal regions of the protein.
(actin-related proteins). While actin and Arp4 are also found in the NuA4
histone acetyltransferase complex, Arp5 and Arp8 are unique to INO80. The
presence of the Rvb proteins, but not their catalytic activity, is required for the
association of Arp5 with the INO80 complex.
96
Arp4, actin, Arp8 (and Taf14)
appear to be associated with an N-terminal region of Ino80.
97
Deletion of
INO80
is lethal in some strain backgrounds and is very sick in others; therefore,
arp5
and
arp8
deletion strains have frequently been used to phenocopy
ino80
deletion (as discussed later).
B. Biochemical Activities
Like other Snf2-remodeling complexes, the ATPase activity of INO80 is
DNA-stimulated, but unlike RSC, INO80 is also capable of 3
0
-5
0
strand dis-
placement in traditional helicase assays.
94
INO80 bound DNA in electropho-
retic mobility shift assays
(EMSAs) and facilitated transcription of a
