Biology Reference
In-Depth Information
TABLE I
S
INGLE
-M
OLECULE
A
NALYSIS OF
P
ROTEIN
M
OVEMENT ON
DNA
MSD (
m
m
2
/s)
Protein
Process
Mode
References
10
3
34
EcoRI
Host restriction
3.5
Sliding
10
2
35
EcoRV
Host restriction
1.1
Sliding and
jumping
10
3
36
EcoRV
Host restriction
3.15
Sliding
10
2
37
Fpg
BER
2.3
Sliding
10
4
-1.3
10
1
38
Lac I repressor
Transcription
2.3
Sliding
10
2
39
Lac I repressor
Transcription
4.6
Sliding
39
Lac I repressor
Transcription
0.4
Sliding
3
a
39
Lac I repressor
Transcription
3D search
10
1
40
Mlh1
MMR
1.37
Hopping (?)
10
1
Hopping
b
40
Mlh1-Pms1
MMR
0.2-9.9
10
2
41
Msh2-Msh6
MMR
1.2
Sliding
10
2
42
MutM
BER
4.0
Sliding
10
2
Sliding/paused
c
43
MutS
MMR
3.6
10
2
37
Nei
BER
3.4
Sliding
10
2
37
Nth
BER
5.8
Sliding
10
1
42
Ogg1
BER
5.8
Sliding
44
PCNA
Replication
1.16
Sliding (direct
and rotational)
10
1
45
p53
DDR
1.62
Complex
10
2
45
p53 (core)
DDR
2.39
Hopping
10
1
45
p53 (C-ter)
DDR
7.76
Sliding
10
2
46
Rad51
Recombination
4.2
1250 bp/s
d
47,48
RecBCD
Recombination
Directed motion
10
3
-4.3
10
1
49
RNAP (T7)
Transcription
6.1
Sliding
10
2
50
RNAP
(
Escherichia
coli
)
Transcription
1.0
Sliding
1.86 (34 mM)
e
51
T7 DNA pol
Replication
Hopping
10
1
51
T7 DNA pol/
thioredoxin
Replication
4
Sliding
10
3
52
Uracil
glycosylase
BER
1.15
Sliding
16
UvrA
NER
None
Jumping
10
4
16
UvrAB
NER
4.4
Sliding
10
3
(
m
m/s)
f
16
UvrAB
NER
1.3
Paused, directed
Conversions:
1
10
3
bp
2
/s
BER, base excision repair; DDR, DNA damage response; Fpg, formamidopyrimidine DNA glycosylase;
MMR, mismatch repair; Nei, endonuclease VIII; Nth, endonuclease III; NER, nucleotide excision repair.
a
3D diffusion constant.
b
D increased with increasing salt, evidence for hopping.
c
short sliding events, followed by long ATP-induced pauses.
d
rate for translocating through lambda DNA.
e
at this ionic strength.
f
directed motion, therefore units
10
15
m
2
/s
10
11
cm
2
10
3
m
m
2
/s
¼
1
¼
1
¼
8.6
¼m
m/s.
