How Clocks and Hormones Act in Concert to Control the Timing of Insect Development - Current Topics in Developmental Biology - page 28

endocrine tissues such as the prothoracic glands would be a fruitful approach
to genetically dissect the requirement of circadian control for hormonal
output.
In the future, the ever-sinking costs of next-generation sequencing
could help to employ RNA-seq in a wide-spanning strategy to obtain highly
reliable tissue-specific expression profiles. This could be done for a range of
animal models, factoring in complex parameters such as different develop-
mental stages, LD vs. DD cycles, gender, zeitgeber times, and different
genetic backgrounds. This and similar high-throughput approaches will ulti-
mately improve our repertoire of circadian-regulated gene networks, and
pave the way for linking clock functions to the regulation of developmental
pathways.
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