Agriculture Reference
In-Depth Information
Rooting of softwood shoot cuttings from
micropropagated (M) and conventionally (C) propagated rootstocks
Table
.
Shoot cuttings
% rooted
Rootstock
Source
Number
after
weeks
'M.
'
M (a)
M (b)
C
'B
'
M
C
'B
'
M
C
'B '
M
C
'B '
M (c)
M (d)
C
'B '
M
C
a, micropropagated plants from a -year-old shoot culture line;
b, micropropagated plants from an -year-old shoot culture
line; c, micropropagated plants from a -year-old shoot culture
line; d, micropropagated plants from a -year-old shoot cul-
ture line. All other micropropagated plants from -year-old shoot
culture lines.
From James
et al.
(). Reproduced with permission.
scion cultivars (Table
), of juvenile as compared with
mature shoots, of etiolated as compared with un-etiolated layer-bed shoots of
apple rootstocks (Doud and Carlson,
.
; Beakbane,
,
), and of wounded or split-based
apple rootstock cuttings as compared with controls (MacKenzie
et al.
,
;
Pontikis
et al.
,
). The effect appears to be on root initiation and differenti-
ation, with a continuous sheath of phloem fibres preventing the development
of outward-pointing meristematic masses such as occur at the lateral margins
of leaf traces or after wounding (MacKenzie
et al.
,
). Wounding also facil-
itates the penetration of IBA (indolebutyric acid) when this is applied to aid
the rooting of cuttings.
!
Wounding is intrinsic to the process of taking cuttings and the production
of roots from a cutting is, at least initially, mainly from tissues close to the
wound. The wound surface can be increased by scoring or splitting the base
