Analysis of Chiral Drugs in Body Fluids - Chiral Separations by Capillary Electrophoresis - page 347

2

3

1

2 mAU

4

(A)

0 5 10 15 20

(B) Migration time (min)

FIGURE 12.3 Electropherograms of a drug mixture obtained by i ber-in-tube SPME-CE (A)
and direct CE analysis (B). 1, desipramine; 2, nortriptyline; 3, imipramine; 4, amitriptyline.
(From Jinno, K. et al., Electrophoresis , 22, 3785, 2001. With permission.)

Polymer-coated
fused-silica iber
(~40 μm diameter)

Te lon rod

Cone-shaped tubing

To detector

Separation capillary
(75 μm id × 360 μm od)

SPME iber assembly

Pt electrode

Bufer reservoir

FIGURE 12.4 Schematic of the SPME-CE interface. (From Whang, C-W. and Pawliszyn,
J., Anal. Commun ., 35, 353, 1998. With permission.)

12.2.2.2 Liquid-Phase Microextraction
LPME is a microextraction technique that was developed from another microextrac-
tion technique called SDME. In SDME the extraction phase is a drop of a water
immiscible solvent suspended from the tip of a microsyringe needle or a tel on rod
in an aqueous sample [19]. After suspending, the drop is immersed in the aqueous
sample and the extraction is performed. After a predetermined time, the drop is