Structure–Function Analysis of Yeast Tubulin - Methods in Cell Biology - page 349

Table 22.1 Plasmids used for genetic manipulation of tubulin in yeast

Linked
prototrophic
marker

Digestion
products
(

Desired
digestion
product (bp)

Plasmid
name

Antibiotic
selection

Digest
using Tubulin gene bp) Reference

pCS6 TUB1 TRP1 Ampicillin Kpn I, Sal I 3500,

4500

4500 Gift from C. Sage

and K. Farrell

pCS3 TUB2 URA3 Ampicillin Sac I, Sph I, 2300,

2500,
5000

5000 Sage, Davis,

Dougherty, Sullivan,
and Farrell (1995)

Pvu II

pMG1 TUB2-6 His URA3 Ampicillin Sac I, Sph I, 2300,

2500,
5000

5000 Gupta, Bode,

Dougherty,
Marquez,
and Himes (2001)

Pvu II

pAFS125 GFP-TUB1 URA3 Ampicillin Stu I 8500 8500 Straight, Marshall,

Sedat, and Murray
(1997)

pMG3 GFP-TUB1 LEU2 Ampicillin Kas I 8500 8500 Gupta et al. (2002)

pMG130 CFP-TUB1 URA3 Ampicillin Stu I 8500 8500 Cederquist et al.

(2012)