Biology Reference
In-Depth Information
FIGURE 19.5
Mass spectrometry. (A) Total ion chromatogram of a PIS experiment at selected m/z values
for control MTs (gray tracing) or ZMP MTs (white tracing). (B) Fragmentation spectrum for
the tubulin-derived tryptic peptides bound to ZMP obtained in a low-resolution triple
quadrupole system. Signals correspond to peptides with four charges. Boxed numbers
correspond to ZMP fragments. The ZMP interaction domain is on the tryptic
-peptide
219-LTTPTYDGLNHLVSATMSGVTTCLR-243. (C) High-resolution MS/MS spectrum
(R
b
30,000) for the triply charged tryptic peptides bound to ZMP acquired on an Orbitrap
Elite mass spectrometer. The asterisks mark the y ions bearing the bound ZMP. Water
losses are labeled with the symbol “o.” Some ions corresponding to the accompanying “a” ion
series are marked with an arrow. The value of z indicates the charge state on each fragment.
Detailed information about the key fragment ions (in the y series) containing the modified
residue (N228 or H229) is shown.
Figures modified from
Field et al. (2012)
with permission from the publisher.
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