Biology Reference
In-Depth Information
FIGURE 19.3
Stoichiometry of MSAs binding to microtubules. (A) Increasing concentrations of
peloruside bound to stabilized taxoid binding sites (10
M). Black circles represent
peloruside found in the pellet; white circles represent peloruside in the supernatant. The
maximum sites available are 10
m
M. Once peloruside has reached this concentration, its
concentration in the pellet remains constant, whereas its concentration in the supernatant
increases relative to the increasing peloruside concentration, indicating 1:1 stoichiometry.
(B) Stoichiometric reaction of increasing ZMP concentrations with stabilized taxoid binding
sites (25 mM). ZMP in the supernatant represents compound that has not reacted with the
taxoid site. ZMP does not appear in the supernatant until at least 25
m
M is present in the
reaction (same concentration as the binding sites). This indicates a 1:1 stoichiometry.
Graph modified from
Field et al. (2012)
with permission from the publisher.
m
