Biology Reference
In-Depth Information
FIGURE 17.5
(A) Light scattering time courses of assembly of 10
M (1) chimera BtubA/
MB
m
b
containing the M loop from
-tubulin in BtubB, (2) wild-type BtubA/B, and (3) calf
b
brain
-tubulin in 80 mM Pipes/NaOH, 1 mM EGTA, 6 mM MgCl
2
, 0.1 mM GMPCPP,
pH 6.8 at 30
C. (B) Corresponding SDS-PAGE electrophoresis, following 2 h assembly
and centrifugation, of BtubA/
ab
MB (5, 10, 15, 20, 25
M), BtubA/B (10, 15, 20, 25, 30
M),
b
m
m
and
M) and controls with 1 mM GDP instead of GMPCPP. (C)
Representative electron micrographs of negatively stained polymers of chimera BtubA/
-tubulin (10
ab
m
b
MB (left, similar to 20
M wild-type BtubA/B) and microtubules assembled from
-tubulin
m
ab
(right). The bar indicates 200 nm.
